Involvement of growth factors in molecular effects of ibuprofen in dental pulp stem cells

Clin Osteol 2022; 27(1): 26-29

Involvement of growth factors in molecular effects of ibuprofen in dental pulp stem cellsReview articles

Adamičková Adriana¹, Adamička Matúš², Gažová Andrea³, Kyselovič Ján^1,4: ² Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University Bratislava, Slovakia; ³ Institute of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Comenius University Bratislava, Slovakia; ⁴ Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia; ¹⁵ th Department of Internal Medicine, Faculty of Medicine, Comenius University Bratislava, and University Hospital, Bratislava - Hospital Ružinov, Bratislava, Slovakia

Stem cells represent promising candidates for regenerative therapy of craniomaxillofacial bone defects, where common techniques, such as autogenous bone graft, allografts or others possess shortcomings and limitations in restoring the morphology and function in bone loss. The efficacy of regenerative therapy with mesenchymal stromal cells (MSC) depends on a combination of the interactions between transplanted MSCs and cellular and molecular components of the recipient, and any current pharmacotherapy in the recipient with effects on transplanted MSC and the bone microenvironment. In the present investigation, dental pulp stem cells (DPSC) were isolated from human impacted third molar teeth. DPSC were treated with ibuprofen in vitro at clinically relevant concentration and relative expression of selected genes were assessed. Our preliminary data suggest a significant effect of ibuprofen as indicated by upregulation of the relative expression levels of growth factors, vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). While the effects of stem cell therapy in bone regeneration are being investigated in ongoing clinical trials, the effects of commonly used pharmacotherapy should be studied for its potential impact on the paracrine effects of stem cells and consequently bone regenerative processes.

Published: June 11, 2022 Show citation

Adamičková A, Adamička M, Gažová A, Kyselovič J. Involvement of growth factors in molecular effects of ibuprofen in dental pulp stem cells. Clinical Osteology. 2022;27(1):26-29.

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