Clin Osteol 2019; 24(1): 27-35

Účinky vitamínu D3, horčíka a vitamínu C na expresiu osteo-asociovaných miRNA mezenchýmových kmeňových buniek tukového tkanivaReview articles

Sabová Nikola1, Samáková Adriana1, Pinček Martin1, Sprušanský Ondrej1, Gažová Andrea2, Kyselovič Ján3
1 Department of Pharmacology and Toxicology, Pharmacy Faculty, Comenius University in Bratislava, Slovakia
2 Institute of Pharmacology and Clinical Pharmacology, Medical Faculty, Comenius University in Bratislava, Slovakia
3 5th Department of Internal Medicine, Clinical Research Unit, Medical Faculty, Comenius University in Bratislava and University Hospital Bratislava - Hospital Ruzinov, Slovakia

Úvod: Vitamín D3, horčík, ale aj vitamín C zohrávajú limitovanú rolu vo vápnikovej homeostáze a fyziológii kostí. Okrem iného majú aj pleiotropné účinky na rôzne typy buniek, akými sú napr. mezenchýmové kmeňové bunky odvodené z tukového tkaniva (Adipose-Derived Mesenchymal Stem Cells -⁠ ADMSCs).


Cieľ štúdie: Cieľom tejto štúdie bolo získať nový pohľad na vplyv magnézia, cholekalciferolu (vitamínu D3) a kyseliny askorbovej (vitamínu C) na potenciálne zmeny v kultivácii a diferenciácii ADMSCs. Nedávny výskum odhalil zapojenie mnohých mikroRNA (miRNA) pochádzajúcich z kostnej hmoty do regulácie fyziológie/patológie kostí. Preto sme sa tiež zamerali na analýzu zmien expresie miRNA v tomto kultivačnom stave.

Metódy: Jednotlivé vzorky ADMSCs sa kultivovali 24 hodín v kompletnom kultivačnom médiu s prídavkom rôznych koncentrácií vitamínu D3, horčíka a vitamínu C. Zmeny v expresii vybraných miRNA kostnej hmoty (miRNA-1, miRNA-29b, miRNA-133a a miRNA-499) sa určili pomocou reverznej transkripcie polymerázovej reťazovej reakcie (Reverse Transcription Polymerase Chain Reaction -⁠ RT-PCR).

Výsledky: Po farmakologickom ovplyvnení buniek pridaním vitamínu D3 sa významne zvýšila expresia všetkých pozorovaných miRNA. Pod vplyvom horčíka bolo zaznamenané štatisticky významné zvýšenie expresie miRNA-1 a miRNA-29b v porovnaní s kontrolnou vzorkou. Zvýšené koncentrácie vitamínu C významne ovplyvnili štruktúru a morfológiu sledovaných buniek, pričom došlo k významným zmenám v expresii miRNA-29b a miRNA-499.

Záver: Táto štúdia potvrdila možnosť pozitívneho farmakologického ovplyvnenia kmeňových buniek vitamínom D3, horčíkom, a taktiež vitamínom C v rámci regulácie expresie miRNA pochádzajúcich z kostnej hmoty.

Keywords: cholekalciferol; mezenchýmové kmeňové bunky odvodené z tukového tkaniva; miRNA; vitamin C; vitamín D3

Published: June 11, 2019  Show citation

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Sabová N, Samáková A, Pinček M, Sprušanský O, Gažová A, Kyselovič J. Účinky vitamínu D3, horčíka a vitamínu C na expresiu osteo-asociovaných miRNA mezenchýmových kmeňových buniek tukového tkaniva. Clinical Osteology. 2019;24(1):27-35.
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